Figure 2 | Scientific Reports

Figure 2

From: Intratumoral heterogeneity of c-KIT mutations in a feline splenic mast cell tumor and their functional effects on cell proliferation

Figure 2

Cell proliferation rate of Ba/F3 cell lines expressing mutant c-KIT proteins. (A) Schematic representation of mutation patterns of c-KIT clones used for this assay. Asterisk indicates the point mutation in exon 10 (M537R) or the point mutation in exons 18 (W854R). The gray box and the blank between exons 9 and 10 indicate the duplicated mutation in exon 8 and the four amino acids deletion in exon 9, respectively. (B) Cell proliferation rate of Ba/F3 cell lines expressing each mutant c-KIT protein. Each mutant cell line was cultured for 48 h in the medium, without (mock, white bar) or with feline SCF (fSCF; gray bar). After 48 h, CCK-8 was added, and the cell proliferation rate was measured by plate reader. Bar graph indicates the mean from four independent biological replicates, and open dots indicate the values obtained from each experiment. p-values were calculated for the comparison among all cell lines using two-tailed one-way ANOVA with a post hoc Tukey–Kramer test. Dagger indicates a significant difference (p < 0.0001) between Mock and fSCF-treated each cell line.

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