Figure 3 | Scientific Reports

Figure 3

From: Intratumoral heterogeneity of c-KIT mutations in a feline splenic mast cell tumor and their functional effects on cell proliferation

Figure 3

Phosphorylation status of c-KIT in Ba/F3 cells expressing the mutant c-KIT. Ba/F3 cell lines stably expressing mutant forms of c-KIT protein were cultured in plain RPMI for 2 h and stimulated with fSCF (100 ng/ml) for 5 min. After stimulation, cells were collected and the extracted whole cell lysates were conducted for SDS-PAGE, followed by western blotting with anti-phospho-c-KIT (Tyr719), anti-Flag (M2), and anti-β-actin antibodies. A representative result of three independent experiments is shown. Full image of western blotting is shown in Supplementary Figure.

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