Figure 4

Shrimp microbiome at phyla and genera level registered at different disease stages. Microbiome modulation for (A) bacteria and (B) fungi taxa. Relative abundance is expressed as a percentage for phyla, where only phyla with higher relative abundance than 1% in at least one of the replicates are shown. Heatmaps using Log2 transformed relative abundance were used to cluster (y axis) genera present in similar conditions. Only genera with higher relative abundance than 1% in at least one of the replicates are shown. Shrimp were grouped (x axis) by the disease stage (early in green, development in red and late in blue) for easy visualization of the microbiome change between healthy and infected shrimp. The heatmaps color key ranges from orange for low abundant (< 1%) microorganisms to cyan for high abundant microorganisms (1–90%). Letters p- (phyla), c- (class), o- (order), f- (family) and g- (genera) on the left side of the taxa name represent the group level.