Figure 5

LEfSe results for enriched taxa of shrimp at early, development a late stage of the disease. Differentially expressed taxa visualized together with heatmap and cladogram graph for bacteria (A,B) and fungi (C,D), respectively. Differentially expressed taxa shown as log10 LDA score for each disease classification. Heatmaps using Log2 transformed relative abundance were used to cluster (x axis) genera by disease stage (early in green, development in red and late in blue). Red squares highlight the differentially expressed taxa in each disease stage condition. Cladograms represent the phylogenetic relation between microorganisms detected at the different disease stages and are marked with a bold alphabetical letter to locate the name of the taxa in the log10 LDA score graph. The heatmaps color key ranges from orange for low abundant (< 1%) microorganisms to cyan for high abundant microorganisms (1–90%). The top 30 overrepresented taxa are shown. Letters p- (phyla), c- (class), o- (order), f- (family) and g- (genera) on the left side of the taxa name represent the group level. For fungi, a subgroup of disease-stage was used: control and D2I from the early-stage, D3E and D4L for the development-stage and D5L for the late-stage. For fungi, subgroups of disease-stage were used: control, D1N, D2N, D2I for early-stage, D3E D3L, D4E and D4L for development-stage and D5E and D5L for late-stage.