Figure 1
From: Importance of the Q/N-rich segment for protein stability of endogenous mouse TDP-43
Schematic diagram of mouse TDP-43 and putative protein structures with various CTR deletions. TDP-43 contains 414 aa residues and consists of a bipartite nuclear localization signal (NLS), two RNA recognition motifs (RRM), and a long-disordered CTR. CTR is divided into four sub-regions, the GaroS1 (yellow), HP (green), Q/N-rich (blue), and GaroS2 segments (purple); the aa sequences are depicted by one-letter codes according to the TDP-43 CTR. The genome-edited mice were categorized into five groups based on the putative CTR structures. Each deleted region is drawn as a red dotted line. The putative aa sequence in each mouse line is shown in black and red letters indicating WT and substituted aa residues, respectively. The gaps in the aa sequences are represented as red dashes. The characteristics of each group determined in this study are shown on the right. Mouse, embryonic lethality of homozygous mice. Protein, protein stability in the eight-week-old mouse cerebrum. mRNA, total expression level of the Tardbp mRNA. NE, not examined. Note that the putative protein structures of ZM93/Δ3 and ZM91/Δ1 mice (gray) are similar to those of ZM13A/Δ9 and ZE10/Δ10, respectively, and were not used in this analysis.
