Figure 2
From: Importance of the Q/N-rich segment for protein stability of endogenous mouse TDP-43
Western blotting and qRT-PCR analysis of the mice at eight weeks of age. (a–c) Cytoplasmic (C), nuclear (N), and insoluble (I) fractions of cerebrum samples from Group 1 homozygous mice (a), Group 3 heterozygous and homozygous mice (b), or Group 4 and 5 heterozygous mice (c) were subjected to western blotting using the antibodies indicated on the right. Anti-α-tubulin and anti-p84 antibodies were used as loading controls for the cytoplasmic and nuclear fractions, respectively. Full-length blots are presented in Supplementary Fig. S4. (d,e) Quantification of λpolyA (external control) and Tardbp mRNA levels normalized to Hprt1 mRNA (internal reference). The total expression levels of Tardbp mRNA were evaluated using Exon 3–4 and Exon 5–6 primer sets. The WT Exon 6 primer set only recognized the WT allele. Each value indicates mean ± SEM as calculated by the REST2009 program. *p < 0.05, **p < 0.01.
