Figure 3
From: Importance of the Q/N-rich segment for protein stability of endogenous mouse TDP-43
Subcellular localization of TDP-43 lacking various CTR deletions, including the GaroS1-HP segment. (a) Immunohistochemical analysis of the spinal anterior horn region in WT and ZM39A/Δ261 heterozygous mice stained with N-260 anti-TDP-43 antibody. Scale bar, 20 μm. (b–d) Transient expression of EGFP-tagged full-length human TDP-43 in HeLa cells. (b) Schematic diagram of full-length human TDP-43 constructs lacking dGaroS1 (aa 262–317), d261CTR (aa 262–346), or dCTR (aa 262–414). (c) Fluorescence and phase contrast images of living cells. Scale bar, 20 μm. (d) Cytoplasmic (C) and nuclear (N) fractions of cell lysates were subjected to western blotting using the antibodies indicated on the right. Note that the blot with the EGFP antibody shows only the low molecular weight region (approximately 27 kDa). Full-length blots are presented in Supplementary Fig. S4.
