Figure 1
Comparison of the characters between miPSCs, miPS-LLCcmP cells and LLC cells. (A) Comparison of the glycerophospholipid composition ratio. (B) Comparison of PGE2 production by ELISA. a: miPSCs, b: miPS-LLCcmP, c: LLC. Data are obtained from three independent experiments and plotted as means ± SD.***p < 0.01. (C) Comparison of relative expression of gene related with PGE2 synthesis. a: miPSCs, b: miPS-LLCcmP, c: LLC. Data are obtained from three independent experiments and plotted as means ± SD. *p < 0.1, **p < 0.05, ***p < 0.01. (D) Comparison of the expression of cPLA2 and COX-2 by Western blotting. A representative blot is shown at left. Densitometric analysis of the blots from three independent experiments by ImageJ is shown at right. a: miPSCs, b: miPS-LLCcmP, c: LLC. Data are plotted as means ± SD. ***p < 0.01. (E) Comparison of the expression of EP-2 and EP-4 by Western blotting. A representative blot is shown at left. Densitometric analysis of the blots from three independent experiments by ImageJ is shown at right. a: miPSCs, b: miPS-LLCcmP, c: LLC. Data are plotted as means ± SD. (F) Time course change of the phosphorylation of Akt in miPSCs. A representative blot is shown at left. Densitometric analysis of the blots from three independent experiments by ImageJ is shown at right. Data are plotted as means ± SD. **p < 0.05, ***p < 0.01. (G) Comparison of the expression of Alox-5 and Lta4h by RT-qPCR. a: miPSCs, b: miPS-LLCcmP, c: LLC. Data are obtained from three independent experiments and plotted as means ± SD. *p < 0.1, **p < 0.05, ***p < 0.01. (H) Comparison of the production of LTB4 by ELISA. a: miPSCs, b: miPS-LLCcmP, c: LLC. Data are obtained from three independent experiments and plotted as means ± SD. *p < 0.1. See details in “Materials and methods” section.
