Figure 2

Evaluation of the effect of PGE2 on miPSCs. (A) Time course change of morphology during 4 weeks of treatment. The images of the cells before treatment are put at the left as the control of the start point with GFP positive cells. BF, bright field; GFP, green fluorescence. Scale bar 50 µm. (B) Flow cytometric analysis for the GFP⁺ population in miPSCs, miPS-LLCcm cells and miPS-PGE2 cells. (C) Comparison of the expression of CD44 and CD133 between miPSCs, miPS-LLCcm cells and miPS-PGE2 cells by RT-qPCR. a: miPSCs, b: miPS-LLCcm, c: miPS-PGE2. Data are plotted as means ± SD. *p < 0.1, **p < 0.05, ***p < 0.01. (D) Comparison of the sphere formation of miPSCs, miPS-LLCcm cells and miPS-PGE2 cells. Scale bar 200 µm. (E) Tube formation assay on miPSCs, miPS-LLCcm cells and miPS-PGE2 cells (left). Scale bar 200 µm. Branching point analysis by ImageJ (right). a: miPS, b: miPS-LLCcm, c: miPS-PGE2. Data are plotted as means ± SD. ***p < 0.01.