Figure 7
Characterization of miPS-PGE2P cells in the presence or absence of PGE2. (A) Adhesive culture. Scale bar 100 µm. (B) Non-adhesive culture. Scale bar 100 µm. (C) Tube formation assay (left). Scale bar 100 µm. Branching point analysis by ImageJ. (right) Data are plotted as means ± SD. There was no significance between the data. (D) Immunofluorescent analyses of tubes in C. BF, bright field. DAPI, staining for nucleus. GFP, fluorescence of GFP. CD31, staining with anti-CD31 antibody labelled with Alexa fluor 555. Scale bars 50 µm (top) and 10 µm (bottom). (E) Western blotting analysis of the phosphorylation of Akt. A representative blot is shown at left. Densitometric analysis of the blots from three independent experiments by ImageJ is shown at right. Data are plotted as means ± SD. *p < 0.1, **p < 0.05, ***p < 0.01.
