Figure 1
From: 3D proximal tubule-on-chip model derived from kidney organoids with improved drug uptake
Proximal tubule epithelial cells in kidney organoids. (a) Schematic overview of process used to create 3D organoid-derived proximal tubule epithelial cell (3D OPTECs)-on-chip models. (b) Confocal image of kidney organoid (tissue-cleared) stained for PODXL+ (red), LTL+ (green), and CDH1+ (magenta), scale bar = 100 μm. (c) Schematic showing marker localization in different segments of the nephron. (d) Higher magnification, confocal image of kidney organoid (cryo-sectioned) stained for LTL+ and CDH1+ regions, scale bar = 10 μm. (e–g) Confocal images of kidney organoid (cryo-sectioned) stained for AQP1+, LRP2+, and SLC3A1+ (magenta) in nephron segments, scale bars = 50 μm. (h) Heat map comparing transporter expression of OPTECs isolated from kidney organoids at days 21, 35, 49, 84, and 105. (i, j) Confocal images of kidney organoid (day 49, cryos-ectioned) stained for OCT2+ (magenta), LTL+ (green), and CDH1+ (cyan), scale bar = 100 μm. (k, l) Higher magnification, confocal images of kidney organoid (day 49, cryo-sectioned) stained for OCT2+ (magenta) and LTL+ (green), scale bar = 10 μm.
