Figure 2
From: 3D proximal tubule-on-chip model derived from kidney organoids with improved drug uptake
Optimizing OPTEC expansion. (a) Brightfield images showing OPTECs grown on plastic, matrigel, and laminin-511 substrates. (b) Heat map showing transporter expression of OPTECs between passages 0 (after isolation) and 5. (c) Brightfield images showing OPTEC populations at passages 0–5, scale bar = 20 μm. (d) Schematic view of the extracellular matrix (ECM), media, and media supplement conditions explored for optimizing OPTEC culture conditions. (e) Heat map comparing transporter expression as a function of the experimental conditions tested in (d).
