Figure 3
From: A preclinical model of cutaneous melanoma based on reconstructed human epidermis
Re-Isolation of single cell suspensions via enzymatic digestion from melanoma and non-melanoma skin models. (A) Schematic of the re-Isolation process of single cells from the epidermal models. (B) Keratinocytes isolated via trypsin from OS-REp as well as melanoma cells isolated via accutase from mOS-REp were stained and quantified on cytospots with markers for keratinocytes (cytokeratin 14) and melanoma cells (Melan-A) (both in red). Cell nuclei were stained with DAPI (blue). Scale bar 50 µm.
