Figure 2 | Scientific Reports

Figure 2

From: Semibulk RNA-seq analysis as a convenient method for measuring gene expression statuses in a local cellular environment

Figure 2

Encapsulation of semibulks with barcoded beads and cross-species validation. (a) A picture of microfluidic device for microdroplet generation. (b) A picture of microdroplets. (c) The distribution of ratio of droplets (containing from zero to three beads or semibulks) fitted with the Poisson distribution. The fitted expected values λ for semibulks and beads were 0.03 and 0.66, respectively. (d) UMI counts, aligned to human and mouse genomes, of the data from the cell mixture. 1451 (47.6%), 1416 (46.4%), and 184 cells (6.0%) were judged as human, mouse cells, and multiplets of these cells, respectively.

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