Figure 2 | Scientific Reports

Figure 2

From: Construction of a culture protocol for functional bile canaliculi formation to apply human iPS cell-derived hepatocytes for cholestasis evaluation

Figure 2

Bile canaliculi formed in suitable culture conditions. Human-induced pluripotent stem cell-derived hepatocytes were cultured in a long-term maintenance medium for 28 days and then, they were sandwich-cultured in a maintenance medium for 9 days. (A) The cell morphology that was observed using a phase-contrast microscope before and after the sandwich culture. The black arrow shows the extended bile canaliculi. After sandwich culture, the excretion of tauro-nor-THCA-24-DBD into a bile canaliculus was observed in a wide area within the well (B: Red dotted line). Fluorescence images show (B) Tauro-nor-THCA-24-DBD, which was accumulated into bile canaliculi.

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