Figure 3 | Scientific Reports

Figure 3

From: Construction of a culture protocol for functional bile canaliculi formation to apply human iPS cell-derived hepatocytes for cholestasis evaluation

Figure 3

Expression of the genes which relate to the maturity of hepatocytes while cells were cultured in a long-term maintenance medium. The expression levels of ALB and AFP were measured using quantitative polymerase chain reaction over time during culture in a long-term maintenance medium for 28 days. The bar shows the relative expression levels of ALB or AFP. Pooled human liver RNA was used for the standard curve, and the expression level was set as one. The relative expression level was calculated using the equation of the line for the standard curve. Data are presented as means ± S.D. (n = 3). ** Shows that expression significantly increased compared with that on day 4 (P < 0.01).

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