Figure 7 | Scientific Reports

Figure 7

From: Construction of a culture protocol for functional bile canaliculi formation to apply human iPS cell-derived hepatocytes for cholestasis evaluation

Figure 7

(A) Microstructure of bile canaliculi was observed using transmission electron microscopy. There are bile canaliculi in the dotted line in the left electron microscopy image. The right electron microscopy image is an enlarged view of the dotted line in the left that shows the microstructure of bile canaliculi. hiPSC-Heps were cultured in the LTM medium for 28 days and then sandwich-cultured in a maintenance medium for 9 days. (B) Accumulation of model fluorescein substrates into bile canaliculi under maintenance or disruption conditions and the microstructure of bile canaliculi. Human-induced pluripotent stem cell-derived hepatocytes (hiPSC-Heps) were cultured in a long-term maintenance (LTM) medium for 28 days and then, they were sandwich-cultured in a maintenance medium for 9 days. Fluorescence images reveal the presence of 5-(and-6)-carboxy-2′,7′-dichloro-fluorescein (CDF), which accumulated into bile canaliculi under maintenance or disruption conditions. hiPSC-Heps were cultured in the LTM medium for 28 days and then sandwich-cultured in a maintenance medium for 9 days.

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