Figure 8 | Scientific Reports

Figure 8

From: Construction of a culture protocol for functional bile canaliculi formation to apply human iPS cell-derived hepatocytes for cholestasis evaluation

Figure 8

Gene expression of cytochrome P450 when the bile canaliculi were formed. Human-induced pluripotent stem cell-derived hepatocytes were cultured in a long-term maintenance medium for 28 days and then, they were sandwich-cultured in a maintenance medium for 9 days. The expression levels of major CYPs were measured using quantitative polymerase chain reaction before and after sandwich culture. The bar shows the relative expression levels of CYPs. Pooled RNA from human liver was used for the standard curve, and the expression level was set as one. The relative expression level was calculated using the equation of the line for the standard curve. The red line shows the maximum value of expression in 22 lots of human cryopreserved hepatocytes. The blue line shows the minimum value of expression in 22 lots of human cryopreserved hepatocytes. Data are presented as means ± SD. (n = 3). ** Shows that expression significantly increased compared with that before sandwich culture (P < 0.01).

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