Figure 4 | Scientific Reports

Figure 4

From: Adjunctive therapy with the Tie2 agonist Vasculotide reduces pulmonary permeability in Streptococcus pneumoniae infected and mechanically ventilated mice

Figure 4

Pulmonary and systemic bacterial burden was not altered by VT therapy 30 h p.i. Vasculotide (VT) (500 ng/100 µl i.v.) or PBS, and ampicillin (0.4 mg/kg bw i.p.) or 0.9% saline were administered 22 h after Streptococcus pneumonia (S. pn.) infection (control: PBS + hyaluronidase). The 6-h ventilation phase began 24 h post infection (p.i). A second dose of VT together with 1 mg HSA was administered 1.5 h prior to termination of the experiment. Bacterial burden in BALF and blood was quantified by plating both samples in increasing dilutions on sheep blood agar, counting CFU and thus determining concentration per µl. (A) Bacterial burden in BALF and in blood (B). Values are listed as median + IQR with minimum/maximum values, individual values are shown as dots (all groups n = 7–8, Mann–Whitney-U-Test).

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