Figure 3

Differentially expressed miRNAs in WB2 vs. WB2.tet mosquitoes. (A) Principal components analysis of log₂ normalised counts of 168 miRNAs. (B) Volcano plots show differential expression of miRNAs in each age group. Red dots represent miRNAs that meet the cut-off for FDR (q) and log₂ fold-change; green, those that meet log₂ fold-change cut-off, but not q significant; blue, those that meet the q significance but not the log₂ fold-change cut-off; grey, those that meet neither cut-off. (C) Venn diagram showing overlap in significantly differentially expressed miRNAs between three age groups: 2 dpe, 6 dpe, and 12 dpe. Only two miRNAs, miR-12-5p and miR-998-5p, were in common between 2 and 6 dpe. A third miRNA, miR-309a-3p, was down-regulated at 2 dpe and up-regulated at 12 dpe. (D) Pairwise comparisons of log₂ fold-changes of miRNAs that were significant in one age group (x-axis) against the same miRNAs in another age group (y-axis). All Pearson correlations were significant (p < 0.05).