Figure 5

Expression of Ae. aegypti miRNAs increasing with age. (A) Venn diagram showing overlap in significantly differentially expressed miRNAs between two mosquito lines. 14 out of 34 miRNAs (41%) were differentially expressed with age in both WB2 and WB2.tet mosquitoes. (B) Graphs showing results of RT-qPCR of six miRNAs that showed significant differential expression between age groups according to RNA-Seq. Points are averages of three biological replicates. Error bars represent standard error of the mean. Significant difference between means was estimated using a factorial ANOVA (p < 0.05). NS, not significant.