Figure 6

Differential expression of selected potential targets of aae-miR-317-3p (top row) and aae-miR-2946-3p (bottom row) that were differentially expressed with mosquito age. RT-qPCR analysis of RNA extracted from mosquitoes at 2, 6, and 12 dpe using primers to the target genes and rps17 as the normalizing gene. Two-way ANOVA with post-hoc multiple comparisons was carried out to compare the expression levels between different time points. Different letters on top of each time point represent statistically significant differences.