Figure 2 | Scientific Reports

Figure 2

From: Adsorption of ice-binding proteins onto whole ice crystal surfaces does not necessarily confer a high thermal hysteresis activity

Figure 2

Purification of PsgIBP. (A) An elution profile of anion-exchange chromatography for the recombination extracts containing PsgIBP. (B) SDS–PAGE electrophoretograms of fractions 1–5 obtained by anion-exchange chromatography. Lane M, molecular weight marker; 0, before chromatography; 1–5, the fractions collected in (A). (C) SDS–PAGE electrophoretograms of purified PsgIBP_S and PsgIBP_L. Lane S, PsgIBP_S; Lane L, PsgIBP_L; Lane A, recombinant AnpIBP (22 kDa) for comparison. The samples were stained with Coomassie brilliant blue R-250. The original gels are presented in Supplementary Fig. 1A and 1B.

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