Figure 3 | Scientific Reports

Figure 3

From: Adsorption of ice-binding proteins onto whole ice crystal surfaces does not necessarily confer a high thermal hysteresis activity

Figure 3

Molecular weight determination for the PsgIBP isoforms based on analytical size exclusion chromatography (SEC). (A) The elution profiles of PsgIBP_S, PsgIBP_L, and reference proteins obtained by using Superose12 gel chromatography. (B) Molecular weight (log10Mw) dependence on SEC elution time. BSA, bovine serum albumin (66 kDa); GFP-Nfe, green-fluorescence protein-labeled notched fin eelpout-derived IBP (34 kDa); AnpIBP, Antarctomyces psychrotrophicus-derived IBP (22 kDa); lpAFP, Longsnout poacher-derived IBP (14 kDa); BpAFP, Barfin plaice-derived IBP (3.5 kDa). On the basis of the calibration line, the MW of approximately 57 and 30 kDa were evaluated for PsgIBP_L and PsgIBP_S, respectively.

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