Table 2 Purification profile of pure pectinase produced by C. parasphaerospermum AUMC 10865 at pH 6.0 and 10 °C in SmF.

From: Production of cold-active pectinases by three novel Cladosporium species isolated from Egypt and application of the most active enzyme

Purification steps

Fraction volume (mL)

Protein (mg/mL)

Total protein (mg)

Activity (U/mL)

Total activity (U)

Specific activity (U/mg)

Yield (%)

Purification factor (fold)

Fermentation medium

1000

0.1464

146.4

0.687

687

4.692

100

1

Ammonium sulfate

6.5

6.46

42

17.31

112.5

2.68

16.37

0.57

DEAE-Cellulose

15.0

0.018

0.27

18.1

271.5

1005.55

39.52

214.4

Sephadex G-100

10.0

0.003

0.03

5.718

57.18

1906

8.32

406.4

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