Table 2 Illumina adapters and primers used in the metabarcoding assay.

From: Assessing a megadiverse but poorly known community of fishes in a tropical mangrove estuary through environmental DNA (eDNA) metabarcoding

 

Oligonucleotide sequence (5′–3′)

References

Adapters

Forward overhang

TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-

[locus-specific sequence]

82

Reverse overhang

GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-

[locus-specific sequence]

Primers

COI

mlCOIintF

GGWACWGGWTGAACWGTWTAYCCYCC

80

jgHCO2198

TAIACYTCIGGRTGICCRAARAAYCA

81

12S

MiFish-U-F

GTCGGTAAAACTCGTGCCAGC

74

MiFish-U-R

CATAGTGGGGTATCTAATCCCAGTTTG

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