Figure 2 | Scientific Reports

Figure 2

From: Alteration of rhesus macaque serum N-glycome during infection with the human parasitic filarial nematode Brugia malayi

Figure 2

MALDI-TOF-MS analysis and HILIC-UPLC profiling of rhesus macaque IgG N-glycans. PNGase F-released glycans from purified rhesus macaque IgG were labeled with 2-AA and analyzed by MALDI-TOF-MS (A) or labeled with procainamide and analyzed using HILIC-UPLC (B). N-glycans were subjected to linkage-specific derivatization of the sialic acids by ethyl esterification prior labeling with 2-AA for MALDI-TOF-MS. MALDI-TOF-MS signals were measured in negative ion reflectron mode and are labeled with monoisotopic masses (see Supplementary Table S2 and Fig. S4 online). N-glycan structures were assigned by combining data from both approaches and by using glycosidase digestions as well as MALDI-TOF-MS/MS (see Supplementary Figs. S5 and S6 online). Proposed structures resulting from this analysis are depicted using the CFG nomenclature (see symbol key inset in Fig. 1). M5 to M9 designate mannosylated N-glycans with 5 to 9 mannose residues.

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