Figure 6
Typical tissue images stained with haematoxylin–eosin (HE) and Goldner's trichrome staining 1 month after surgery. HE staining of bone tissue in controls A1, C1, E1, and G1 and B1 and F1 at the negative electrode connection shows the formation of numerous trabeculae with no gaps between the tissues. Bone tissue D1 and H1 at the positive electrode connection shows limited trabecular formation with some areas showing neither trabeculae nor osteocytes. Goldner staining of bone tissue in controls A2, C2, E2, and G2 and at the negative electrode connections B2 and F2 shows large areas of green mineralised bone that is connected in sheets with only a small amount of bone-like material with no gap between the tissues. Bone tissue at the positive electrode junction D2 and H2 shows a large area of red osteoid at the positive electrode fracture with very little green mineralised bone (N ≥ 3).
