Figure 3
hTERT expression in Jurkat cells exosomes and in subsequent fibroblasts. A. Exosomal RNA was isolated from three different exosome isolations. hTERT and HPRT-1 as a control gene were amplified by Real-Time PCR. The Y axis represents the normalized hTERT expression level in Jurkat cells exosomes compared to control fibroblasts exosomes. For calculation needs, a threshold of Ct 50 was used as the "undetectable" hTERT expression in fibroblasts. The RQ values were calculated based on the 2\(\Delta \Delta\)ct and were multiplied by 100. The bars represent average hTERT expression of each sample ± S.E of three independent experiments. B. 3 × 105 pHFF cells/ml cells were subjected to Jurkat-derived exosomes for 6 h. The cells were harvested and the expression of the hTERT mRNA was carried out by quantitative real time PCR. The control cells are those that were exposed to PBS only. Each column represents mean ± S.E of three independent experiments. Exosomes: cells exposed to Jurkat derived exosomes, control- cells exposed to PBS only.
