Figure 1
From: PGC-1β maintains mitochondrial metabolism and restrains inflammatory gene expression
Comparing metabolite and gene expression profiles of metabolically distinct DCs. DCs were stimulated with LPS (100 ng/mL) or IFN-β (1000 U/mL) for 18 h and the (a) OCR (left) and ECAR (right) were measured by Seahorse bioanalyzer over time with sequential treatments with oligomycin (oligo), FCCP, and antimycin and rotenone (anti/rot). (b) Volcano plot of data significance (-log10(p)), with a dotted line at p = 0.05, versus log2(fold change) for changes in metabolite relative abundance in LPS-activated DCs (left) and IFN-β-treated (right) compared to unstimulated DCs. (c) Heat maps of expression of genes related to KEGG pathways of glycolysis/gluconeogenesis, tricarboxylic acid cycle, and fatty acid metabolism, by unstimulated DCs, or DCs activated with LPS or IFN-β for 6 h. (d) Raw counts from RNA sequencing data of Ppargc1b and Ppargc1a. Data in (a) are of one experiment representative of at least three experiments (mean and s.d. of five replicates).
