Figure 4
From: Differential transcriptomic responses to heat stress in surface and subterranean diving beetles

Phylogenies of heat shock Trinity ‘genes’ inferred using RAxML to validate the identity of putative HSP orthologs in Paroster species. Trees are as follows: (A) sHSP family, (B) HSP60, (C) HSP90, (D) HSP70. Scale bar is in substitutions/site; BS = bootstrap node support. Tip labels show transcript names/GenBank accession numbers. Tip names for P. macrosturtensis are shared with the reference P. nigroadumbratus transcript reads were assembled against. Tip names with stars specifically refer to genes differentially expressed in the presence of the experimental setup alone relative to the control (comparison 1) or at 35 °C relative to 25 °C in the experimental setup (comparison 2).