Figure 2 | Scientific Reports

Figure 2

From: DNA walking system integrated with enzymatic cleavage reaction for sensitive surface plasmon resonance detection of miRNA

Figure 2

(A) Gel electrophoresis characterization of the TSDR. Lane 1: 2 μM blDNA; Lane 2: 1 μM swDNA; Lane 3: 1 μM swDNA/2 μM blDNA duplex; Lane 4: 2 μM miRNA-182; Lane 5: 1 μM swDNA/2 μM blDNA duplex + 2 μM miRNA-182. (B) SPR sensorgrams upon injection of 50 nM SA onto the Nb.BbvCI-treated fluidic channels that were covered with (a) trDNA + swDNA/blDNA duplex; (b) trDNA + swDNA/blDNA + miRNA-182; (c) trDNA + swDNA; (d) non-biotinylated trDNA + swDNA/blDNA duplex + miRNA-182.

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