Figure 5
Cell viability of 661W cells cultured under stress-free conditions with an endothelin antagonist or agonist. The relative amount of live cells was measured using WST-8 following 48 h treatment with or without bosentan (250, 500 or 1000 nM) and with or without endothelin-1 (1, 10 or 100 nM). (a) Quantitative analysis of live cells (n = 3, for all treatments). NS: no significant difference vs. without bosentan nor endothelin-1, Tukey’s HSD. B: bosentan, E: endothelin-1. (b–d) Representative images of 661W cells cultured under each condition. Bar: 50 µm.
