Figure 6
Cell viability of 661W cells cultured under glucose-free conditions with an endothelin antagonist or agonist. The amount of live cells was measured using WST-8 following 48 h treatment in glucose-free media with or without KUS121 (100 µM), with or without bosentan (100; 500; 2500 nM), and with or without endothelin-1 (1; 10; 100 nM). (a,b) Quantitative analysis of live cells (n = 3, for all treatments). 661W cells were cultured under glucose-free conditions, with (b) or without KUS (a), with or without bosentan (100; 500; 2500 nM), and with or without endothelin-1 (1; 10; 100 nM). NS: no significant difference, vs. glucose-free without bosentan nor endothelin-1, Tukey’s HSD. *p < 0.05, **p < 0.01, ***p < 0.001, vs. no glucose with KUS, Tukey’s HSD. K121: KUS121, B: bosentan, E: endothelin-1. (c–h) Representative images of 661W cells cultured under each condition. Bar: 50 µm.
