Figure 7
Cell viability of 661W cells under endoplasmic reticulum stress. Cells were analyzed following 40 h treatment with tunicamycin (TM, 0.2 µg/mL) and with or without KUS121 (100 µM). (a) Quantitative analysis of live cells (n = 3, for all treatments). 661W cells were treated without TM and without KUS121 [labeled ‘‘(-)’’], with TM and without KUS121 (control, labeled ‘‘C’’), or with TM and KUS121 (labeled ‘‘K121’’). **p < 0.01, ***p < 0.001, Tukey’s HSD. (b–d) Representative images of 661W cells cultured under each condition. (e,f) Quantitative analysis of live cells (n = 3, for all treatments). 661W cells were treated with TM and with (f) or without KUS121 (e) with bosentan (0; 100; 500; 2500 nM) or endothelin-1 (0; 1; 10; 100 nM). NS no significant difference, vs. control (without bosentan nor endothelin-1) in (e), vs. KUS121 (without bosentan nor endothelin-1) in (f), Tukey’s HSD. K121: KUS121, B: bosentan, E: endothelin-1. (g–l) Representative images of 661W cells cultured under each condition. Bar: 50 µm.
