Figure 2

Protein expression of total cTnI and phosphorylation of cTnI at Ser23/24 in non-diabetic and diabetic rat left ventricular tissues. (a) Representative immunoblots of total cTnI, phosphorylated cTnI Ser23/24 (cTnI pSer23/24), and GAPDH in non-diabetic and diabetic rat left ventricular tissues. Quantification of (b) total cTnI/GAPDH, (c) phosphorylated cTnI Ser23/24 / GAPDH, and (d) phosphorylated cTnI Ser23/24/total cTnI levels in non-diabetic and diabetic rat left ventricular tissues. Data shown are means ± SEM, obtained from duplicate blots, *P < 0.05 versus non-diabetics, unpaired t-test. Original immunoblots are presented in Supplementary Fig S1.