Figure 2

Allelic discrimination graph of 13104-probe qPCR assay for AA, AB, BB, BE and EE genotyped DNA samples from hair follicles of genotyped animals. Nonspecific amplifications were not observed. The Blue dots represent BB genotype (HEX reporter), the greens dots represent heterozygous AB or BE genotypes, the yellow dots represent the heterozygous AE and the homozygous AA and EE genotypes (FAM reporter); the black dots represent negative control (NTC). A, B and E synthetic reference samples were used as a positive control of the assay. Data were plotted using RFU of the FAM reporter dye of A and E alleles on the x axis and using RFU of the HEX reporter dye of B allele on the y axis. RFU = relative fluorescence units.