Table 3 Schematic representation of the expected results of duplex qPCR assays with each reporter dye in the 13104 and 13124 positions.

From: Development of a duplex qPCR assay with locked nucleic acid probes for A, B and E kappa-casein variants detection

Position

Reporter

Ƙ-Casein genotype

AA

AB

AE

BB

BE

EE

131041

FAM

✔

✔

✔

 

✔

✔

HEX

 

✔

 

✔

✔

 

131242

Cy5

✔

✔

✔

✔

✔

 

FAM

  

✔

 

✔

✔

  1. 1The 13104_C probe for B detection was labelled with HEX reporter dye and the 13104_A probe for A and E detection was labelled with FAM reporter dye.
  2. 2The 13124_G probe for E detection was labelled with FAM reporter dye and the 13124_A probe for A and B detection was labelled with Cy5 reporter dye.
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