Figure 1
From: Multicolor multifocal 3D microscopy using in-situ optimization of a spatial light modulator
Example images showing the effect deploying an SLM-displayed multifocal grating pattern for an emission filter for which the pattern is not designed for. Here, an SLM pattern is displayed which was optimized for a \(3\times 3\) subimage array using an emission filter having \(\lambda _\text {c}=671\) nm and SLM calibration settings giving \(0-2\pi\) relative phase control for \(\lambda = 696\) nm. Using this pattern, corresponding bright-field mode multifocal images are obtained using emission filters having (a) \(\lambda _c=671\) nm, which is the pattern design wavelength giving \(M=0.81\), and (b) \(\lambda _c=512\) nm, which is far from the design wavelength giving \(M=0.07\). The orange rectangle indicates the camera region occupied by the central \(3\times 3\) subimage array.
