Figure 5 | Scientific Reports

Figure 5

From: Multicolor multifocal 3D microscopy using in-situ optimization of a spatial light modulator

Figure 5

Example multifocal images from a sequence of two-color imaging of a mixture made up of yellow-green (505/515) and red (580/605) \(\mu\)m-sized fluorescent particles. (a) \(\lambda _\text {c}=512\) nm channel, and (b) \(\lambda _\text {c}=610\) nm, (c) 3D rendering of the combined multicolor multifocal images (a) and (b) showing the \(\lambda _\text {c}=512\) nm channel particles in green and \(\lambda _\text {c}=610\) nm in red. The black tail of each particle indicates the corresponding moving trajectory. The scale bar in each figure represents 20 \(\mu \hbox {m}\).

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