Figure 6
From: Multicolor multifocal 3D microscopy using in-situ optimization of a spatial light modulator
Example multifocal images from a sequence of two-color imaging of a sample consisting E. coli and human liver cells. (a) \(\lambda _\text {c}=512\) nm channel, and (b) \(\lambda _\text {c}=671\) nm, (c) overlay of the central subimages corresponding to \(z=0\) plane from both (a) and (b). The E. coli is colored green while the human liver cells are colored red, (d) 3D rendering of the images in (a) and (b) showing the mammalian cells (red) and the E. coli cells (blue). The scale bars in (a) and (b) represent 20 \(\mu\)m, the scale bar in (c) represents 5 \(\mu \hbox {m}\).
