Figure 4

The effects of COS on the development of tubulointerstitial fibrosis in UUO-induced RF mice. Representative micrographs illustrating of H&E and Masson trichrome staining from kidney tissues (A). The tubulointerstitial injury score based on H&E staining (B). Percentage of the Masson trichrome-positive tubulointerstitial area relative to the entire area (C). The different colored arrows in Fig. 5A indicated the following meanings. The Black arrows implied necrosis of the tubular epithelial cells or hyperchromatism or disintegration of the nucleus or increased eosinophilic cytoplasm. The red arrows represented structural damage to the glomerulus or congestion of capillaries in the glomerulus. The yellow arrows showed inflammatory cells oozing out. The green arrows meant the epithelial cells of the renal tubules are edema and swollen, and the cytoplasm is loose, stained or vacuolar. All values are represented as mean ± SD (n = 10 in each group). Superscript letters a and b designate significant differences. ^aP < 0.05 compared with the model group. ^bP < 0.01 compared with the model group.