Figure 1
From: Isolation and characterization of a Vibrio owensii phage phi50-12
Pathogenicity of V. owensii GRA50-12 was confirmed by using zebrafish as a model. (a) Three groups (8 fishes/group) were considered, and their survival rates were measured after intraperitoneal injection of V. owensii GRA50-12 cells (2.2 × 106 CFU/20 µl, black line; 2.2 × 104 CFU/20 µl, red line) or PB3S (PBS with 3% NaCl) as control (green line). The X-axis represents the time post-infection and the Y-axis represents the survival percentage of the zebrafish. (b) The disease symptoms in V. owensii GRA50-12 infected zebrafish. Control represents PB3S buffer injected fish; thus, no symptoms were observed. 50-12 represents zebrafish injected with GRA50-12 and these showed swollen abdomen and bleeding at body surface (left: side view; middle: top view; right: abdomen anatomy). The anatomical analysis showed swollen and bleeding intestines. The significance of the differences between groups was performed by Log-rank and Gehan–Breslow–Wilcoxon test in GraphPad Prism 9. “*” indicates p < 0.05.
