Figure 1 | Scientific Reports

Figure 1

From: Identifying a novel role for the master regulator Tal1 in the Endothelial to Hematopoietic Transition

Figure 1

The simultaneous overexpression of Runx1, Cbfb, Gata2, Erg and Fli1, but not that of Tal1, Lmo2 and Lyl1 generates a hemangioblast culture enriched in cells resembling Pre-HPCs. (A) Scheme of the mESC differentiation system used in the study showing the cell-surface markers used to identify the different cell populations. (B) Schemes describing the transgenes used in the study. T2A = viral T2A peptide sequences inserted between the coding sequences of the genes to exploit the “ribosomal skipping” mechanism for the production of all proteins from one single transcript. (C) FACS analysis results showing the expression of VE-CAD and CD41 in day 3 hemangioblast cultures from i5TFs and i3TFs mESCs treated with doxycycline at day 1 of culture. Left: representative FACS plots. Right: Box plots summarizing the results of 3 experiments. Significance was determined by Analysis of Variance (ANOVA) test. Error bars correspond to standard deviations.

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