Figure 2 | Scientific Reports

Figure 2

From: Identifying a novel role for the master regulator Tal1 in the Endothelial to Hematopoietic Transition

Figure 2

The simultaneous overexpression of the 5TFs transdifferentiates sorted eVSM cells into VE-CAD+CD41+ cells less efficiently compared to the 8TFs. (A) Experimental layout of the work. (B) Representative microscopy images of the indicated conditions at day 2.5 HE culture. VSM cells appear as large wide-spread cells; V+CD− cells appear as elongated cells; HPs appear as round floating cells. The white box highlights an example of the endothelial cell cluster from which hematopoietic cells normally arise. (C) Flow cytometry analyses of the indicated conditions. The squares highlight the populations sorted for cell culture and molecular biology. (D) Box plots showing the relative frequencies of VSM, V+CD−, V+CD+, and HP populations in HE culture from i8TFs, i5TFs and i3TFs lines (n = 3). Significance was determined by Analysis of Variance (ANOVA) test. Error bars correspond to standard deviations.

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