Figure 5
3TFs overexpression contributes to the silencing of the muscle transcriptional program and the activation of the hematopoietic one in eVSM. (A) Heatmap showing the expression of genes from the clusters indicated in the figures. The most representative GO terms enriched in each cluster are shown in the left. * KEGG enrichment (no significant GO terms could be found for cluster 7). (B) diffTF volcano plot comparing i3TFs untreated (unt) eVSM to i3TFs dox-treated (dox) eVSM. A detailed explanation of the plot can be found in Fig. 3. TFs characteristic of VSM cells and hematopoietic TFs are circled. (C) Results of the “Known Motif Enrichment” analysis performed with the HOMER software on the differentially expressed genes in i3TFs dox eVSM. The top 5 enriched transcription-factor motifs are shown. The Tal1 motif is highlighted in orange. (D) Selection of differentially expressed genes (DEGs) comparing i3TFs unt eVSM to i3TFs dox eVSM. Hematopoietic genes are shown in pink, VSM-related genes are shown in blue. (E) diffTF volcano plots comparing i8TFs V+CD+ to i5TFs V+CD+ (left panel) and i8TFs dox V+CD+ to i5TFs dox V+CD− (right panel). A detailed explanation of the plots can be found in Fig. 3. VSM and hematopoietic TFs are circled.
