Table 1 Factors and levels used in the MAE experimental design of bioactive components from carrot rejects.
Factors | Levels | |
|---|---|---|
Experiment 1: Preliminary experiments for solvent selection | ||
Solvent type | 8 Solvents (polar solvents and solvent mixture of polar and non-polar) | |
Ethanol, Ethyl acetate, Acetone, Hexane, Hexane/ethanol (1:1), Hexane/ethyl acetate (1:1), Hexane/acetone (1:1), Hexane/acetone/ethanol (2:1:1) | ||
Experiment 2: Half Factorial design (24–1 factorial points + 3 center points) | ||
Solvent type (Hexane:Ethanol) | 25 | 100 |
Temperature (°C) | 40 | 70 |
Time (min) | 5 | 15 |
Solid:Solvent ratio (g/mL) | 1:20 | 1:40 |
Experiment 3: Rotatable CCD design for microwave digester process parameters optimization (FCCD with 3 factors and 5 levels + 5 center points) | ||
Temperature (°C) | 40 | 60 |
Time (min) | 5 | 15 |
Solid:Solvent ratio (g/mL) | 1:20 | 1:40 |
