Figure 2
Overexpression of ACSL1 mediates imatinib-induced senescence in K562 cells. (a,b) qRT-PCR and western blot analysis verified efficiency of overexpressing ACSL1. (c) Cell senescence analysis determined by SA-β-gal staining. (d,e) qRT-PCR was applied to quantify the relative expression of senescence-associated genes p21 and IL-6. (f) Cell proliferation analysis determined by CCK8 assay. (g) Cloning formation assay indicating the in vitro growth of K562 cells. (h) Cell cycle distribution analyzed using PI staining followed by flow cytometry. (i) Representative images of tumor from a Xenograft model in nude mice. (j) Tumor weight of all mice in each group. (k) Tumor volumes were measured on the indicated days. The data are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001.
