Figure 3
Knockdown of ACSL1 expression reverses imatinib-induced senescence in K562 cells. (a,b) Cells were transfected with si-NC or si-ACSL1. (c) Cell senescence analysis determined by SA-β-gal staining. (d,e) qRT-PCR was applied to quantify the relative expression of senescence-associated genes p21 and IL-6. (f) Cell proliferation analysis determined by CCK8 assay. (g) Cloning formation assay indicating the in vitro growth of K562 cells. (h) Cell cycle distribution analyzed using PI staining followed by flow cytometry. The data are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001.
