Figure 2 | Scientific Reports

Figure 2

From: Diesel exhaust particles induce human umbilical vein endothelial cells apoptosis by accumulation of autophagosomes and caspase-8 activation

Figure 2

DEP initiate autophagy in HUVECs. (a) HUVECs were exposed to DEP (70 µg/ml) for the indicated times and phosphorylation of Beclin-1 at Ser93 was analyzed by immunoblotting. (b) Quantification of Beclin-1 phosphorylation normalized to total Beclin-1. Results are presented as means ± SD (n = 3). Statistical analysis was performed using one-way ANOVA. **P < 0.02 versus no treatment. (c) HUVECs were exposed to DEP (70 µg/ml) for the indicated times and protein levels of p62 and LC3B were measured by immunoblotting. (d) Quantification of p62 and LC3BII protein levels normalized to ⍺-tubulin. Results are presented as means ± SD (n = 3). Statistical analysis was performed using one-way ANOVA. *P < 0.05 versus no treatment. **P < 0.02 versus no treatment. (e) HUVECs infected with GFP-LC3B-expressing adenovirus were exposed to DEP (70 µg/ml) for 18 h and formation of LC3B puncta was examined with fluorescence microscopy. (f) Quantification of LC3B puncta formation per cell. Results are presented as means ± SD (n = 10). Statistical analysis was performed using two-tailed Student’s t test. **P < 0.02 versus control.

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